Nucleolar proteins B23 and C23 as target antigens in chronic graft- versus-host disease

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Nucleolar proteins B23 and C23 as target antigens in chronic graft-versus-host disease.

Previously we observed that sera from recipients of allogeneic bone marrow transplants who developed extensive chronic graft-versus-host disease (GVHD) intensively stained the nucleolar region of target cells in indirect immunofluorescence microscopy. To identify the target antigens, immunoblotting experiments were performed using isolated nuclei, isolated nucleoli, and purified nuclear and nuc...

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Target antigens determine graft-versus-host disease phenotype.

Chronic graft-vs-host disease (cGVHD) is an increasingly frequent complication of allogeneic stem cell transplantation. Phenotypically, cGVHD differs from patient to patient; in particular, a subset of patients develops extensive cutaneous fibrosis. Similarly, graft-vs-host disease (GVHD) is distinct in inbred murine donor:recipient pairings, indicating a genetic component to disease phenotype....

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cDNA sequences of chicken nucleolin/C23 and NO38/B23, two major nucleolar proteins.

Nucleolin and NO38 are abundant nucleolar proteins implicated in the transcription and processing of ribosomal RNA, and in the packaging and transport of ribosomal proteins or preribosomal particles (for references see 1-5). cDNAs encoding the chicken homologs of these proteins were cloned (by lmmunoscreening; 6) from Xgtll cDNA expression libraries prepared from poly(A)+ RNA of 10 day old chic...

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Chronic graft-versus-host disease.

Mr. F is a 62-year-old Caucasian man who was diagnosed with acute myelogenous leukemia (AML) 15 months prior. Following two cycles of induction chemotherapy, his AML was in remission. Four months ago, after a reduced-intensity conditioning regimen, Mr. F received an allogeneic peripheral blood stem cell transplant from his human leukocyte antigen–identical 56year-old sister. Mr. F received a re...

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Progress in chronic graft-versus-host disease

255 PRF deficiency occurs not only with allelic variants characterized by mutations in the coding region of the gene. McIlroy et al. reported that the occurrence of a C/T polymorphism at position-398 of the proximal promoter region of the PRF1 gene was associated with decreased quantities of PRF in CTLs in HIV infected patients. A decrease of about 50% of control PRF levels was observed in hete...

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ژورنال

عنوان ژورنال: Blood

سال: 1992

ISSN: 0006-4971,1528-0020

DOI: 10.1182/blood.v79.4.1081.bloodjournal7941081